Coding

Part:BBa_K1677102:Design

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-20)


Invasin with Usp45 Signal Peptide (Lactococcus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
    Illegal BglII site found at 878
    Illegal BglII site found at 2318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2347
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled using gibson assembly to join 5 five overlapping IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis.

Source

The invasin gene was originally source by the Warsaw 2010 team from the bacterium Yersinia something or other.

References