Coding
Part:BBa_K1677102:Design
Designed by: Mackenzie Labine-Romain Group: iGEM15_BABS_UNSW_Australia (2015-08-20)
Invasin with Usp45 Signal Peptide (Lactococcus)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 794
Illegal BglII site found at 878
Illegal BglII site found at 2318 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2347
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was assembled using gibson assembly to join 5 five overlapping IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis.
Source
The invasin gene was originally source by the Warsaw 2010 team from the bacterium Yersinia something or other.